Flexible Microfabrication on a Chip during Cultivation for a Neuronal Network Direction Control Using Stepwise Photo-Thermal Etching of an Agarose Architecture

Control over spatial distributions and patterns of individual neurons their neurites provides an essential tool for studying the meaning neuronal network patterns. Moreover, complete direction control synaptic connections between cells in each is also to investigate detailed information on relationship forward feedback signaling among cells. Here, we have developed a method topographical within living using new type individual-cell-based on-chip cell-cultivation system with agarose microfabrication technology. The advantages this include ability positions number cultured cells, as well flexible elongation axons dendrites stepwise melting thin layer coated cultivation chip focused infrared laser beam even during without any destructive damage Using system, succeeded forming fully direction-controlled single-cell-based from rat hippocampal In meeting, discuss potential heat demonstrate our cell-based neural networks.